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Primers used to amplify the 3ABC and 2C genes
| Primer name | Primer sequencea |
|---|---|
| 3ABC-F | 5′-CGGGATCCATCTCAATTCCTTCCCAAAAGTCC-3′ |
| 3ABC-R | 5′-CCGCTCGAGTCTCATGGTGTGGTTCGGGGT-3′ |
| 3C-46-F | 5′-CGTACCTCGTTACCTTTTCGC-3′ |
| 3C-46-R | 5′-GCGAAAAGGTAACGAGGTACG-3′ |
| 3C-163-F | 5′-AGGCTACGGTGGGGGAGC-3′ |
| 3C-163-R | 5′-GCTCCCCCACCGTAGCCT-3′ |
| 2C-R | 5′-CCGCTCGAGCTGCTTGAAAATCGGGTG-3′ |
| 2C-F | 5′-CGGGATCCCTCAAAGCACGTGACATCAA-3′ |
The restriction enzyme sites are underlined.
