CD3+ CD4+ T helper cell and CD3+ CD8+ cytotoxic T cell frequencies as a percentage of total MNCs in the blood, spleen, ileum, and duodenum in deficient and sufficient HIFM/HRV pigs at PID14, determined by flow cytometry. (A) CD3+ CD4+ T helper cells. (B) CD3+ CD8+ cytotoxic T cells. Results are ±standard errors of the means (SEM). *, significant (P < 0.05) change in deficient HIRM/HRV pigs compared to sufficient HIFM/HRV pigs (Mann-Whitney test).