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. 2017 Aug 31;83(18):e01013-17. doi: 10.1128/AEM.01013-17

FIG 3.

FIG 3

Nucleotide incorporation efficiency of dCTP incorporation onto 15-mer/TG*. A preincubated reaction mixture of wtDpo4 or mutants (120 nM) and 15-mer/TG* (30 nM) was rapidly mixed with dCTP (0 to 400 μM) for various times before being quenched. (A) Dependence of product concentration on time was plotted and fit to equation 1 (see Materials and Methods) to determine kobs. (B) kobs was plotted against dCTP concentration, and data were fit to equation 2 (see Materials and Methods), which determined a kp of 0.062 ± 0.009 s−1 and a Kd,dCTP of 148.40 ± 18.36 μM.