FIG 2.

Phylogenetic analyses of SFB FliC3 and FliC4 genes at the nucleotide and deduced amino acid levels. Fifty-two rat SFB fliC3 gene sequences, 42 rat SFB fliC4 gene sequences, 66 mouse SFB fliC3 gene sequences, and 51 mouse SFB fliC4 gene sequences were obtained by clone library sequencing. For each sample, duplicate sequences were deleted, and one sequence was preserved for further analysis. Deduced amino acid sequences shorter than half the length of the previously reported SFB fliC genes were removed. In total, 201 fliC3 and fliC4 nucleotide sequences and 130 deduced FliC3 and FliC4 amino acid sequences were used for phylogenetic analysis. Three mouse SFB fliC3 sequences, 3 mouse SFB fliC4 sequences, 1 rat SFB fliC3 sequence, and 1 rat SFB fliC4 sequence were downloaded from the NCBI GenBank database. Finally, 209 fliC3 and fliC4 nucleotide sequences (A) and 138 deduced amino acid sequences (B) were aligned and then used to construct a phylogenetic tree by using MEGA 6 with the neighbor-joining algorithm.