Figure 3.

Detection of virus shedding. Biological samples, including nasal, oral, and rectal swabs, were collected at indicated time points after inoculation with VSV-IFNβ-NIS. RNA from biologic samples were analyzed by qRT-PCR to detect VSV nucleocapsid (N) RNA, indicating that low levels of viral RNA were detectable in (A) oral, (B) nasal, and (C) rectal swabs in directly inoculated pigs (Nos. 45408–45411) but not in contact pigs (45412–45415). No infectious virus was detectable in any collected samples.