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. 2017 Aug 30;11:20. doi: 10.3389/fnint.2017.00020

Figure 2.

Figure 2

Excitatory neurons in the mitral cell layer of the accessory olfactory bulb (AOB) generate col23a1 mRNA. (A) Schematic depicting the location and layers of the AOB. GL, glomerular layer; MCL, mitral cell layer; GCL, granule cell layer. (B) In situ hybridization (ISH) for col23a1 mRNA revealed layer-specific expression in the AOB (arrow). Sporadic expression was also observed in the MCL of the main olfactory bulb (MOB; arrow heads). (C) ISH coupled to immunostaining for VGluT2 or Calr, revealed expression of col23a1 in the MCL of the AOB. (D–F) Double ISH with riboprobes against col23a1 and either syt1 or gad1, revealed excitatory, and not inhibitory, neurons generate this MACIT. (E,F) depict high magnification images of col23a1 expression in syt1-expressing neurons, but not in gad1-expressing interneurons. Scale bar in (B) = 400 μm, in (C) = 200 μm, in (D) = 200 μm and in (E) and (F) = 50 μm.