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. 2017 Aug 31;8:1047. doi: 10.3389/fimmu.2017.01047

Figure 6.

Figure 6

UCB CD5− ILC2s are functionally competent and derive from CD5+ ILC2s. (A) Cytokine production of bulk CD5+ ILC2 after 2 weeks of coculturing with the irradiated allogenic peripheral blood mononuclear cells and JY cell (feeder cells) with IL-2 (100 U/ml) and IL-7 (10 ng/ml). Total cells were stimulated with P/I for 6 h. (B) qPCR analysis of cytokine expression level of CD5+ ILC2 derived CD5+ ILC2s and CD5- ILC2s. Cells were sorted from the feeder cells coculture with IL-2 (100 U/ml) and IL-7 (10 ng/ml) after 2 weeks and unstimulated or stimulated with P/I for 6 h (n = 5). *P < 0.05 (Student’s t-test) (C) Single CD5+ CRTH2+ ILC2 cell was sorted and cultured in feeder cells with IL-2 (100 U/ml) and IL-7 (10 ng/ml) for 2 weeks and cytokine production was evaluated after stimulated with P/I for 6 h. Counter plot (left top): CD5+ CRTH2+ ILC2 (red), CD5−CRTH2+ ILC2 (blue) and CD5− CRTH2− ILC1 (black). Dotplot (right top): CD5 and CD161 expression on one representative clone (4G11). Histogram: clone (red), isotype control (gray filled), and IFN-γ positive control (black dashed). Pie chart indicate the frequency of clones producing IL-5 and IL-13 or IL-5 and IL-13 and IL-22.