Skip to main content
. Author manuscript; available in PMC: 2017 Sep 5.
Published in final edited form as: Science. 2015 Dec 10;351(6269):186–190. doi: 10.1126/science.aad0512

Fig. 4. Identification of an autoinhibitory interaction site within CCL21.

Fig. 4

(A) Migration of mature BMDCs within 3D collagen gels towards truncated CCL21 (CCL21trunc). All graphs depict average speed in y-direction ±SD over time of 8 independent experiments with cells generated from at least three different mice. (B) (left) Overlays of a portion of the 15N-1H HSQC spectra of CCL21-FL 1–111 (black) and CCL21trunc 1–79 (purple). (right) The first 79 amino acids of the CCL21-FL structure are depicted with residues showing significant changes in chemical shift perturbations upon truncation. Red circle indicates putative autoinhibitory site. (C) (left) Overlays of a portion of the 15N-1H HSQC spectra of CCL21-FL 1–111 (black) and CCL21-FL titrated with increasing concentrations of polySia DP9 (grays to cyan). (right) Residues within CCL21-FL with significant polySia induced chemical shift perturbations are colored cyan. (D) (right) Schematic representation of chemokines used for in vitro migration assays. FL: full length. (left) Migration of mature BMDCs towards the chimeric chemokine.