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. Author manuscript; available in PMC: 2017 Sep 5.
Published in final edited form as: Anal Chem. 2017 May 25;89(12):6330–6335. doi: 10.1021/acs.analchem.7b01048

Figure 1. Acid-catalyzed hydrolysis of sialic acids in dimethyl sulfide (DMSO)-acetic acid (AA).

Figure 1

(a) Hydrolysis of native sialic acid (N2H2S2) (Sialylglycopeptide; SGP) in DMSO-AA at 4°C, 23°C, 37°C, and 65°C; (b) p-Toluidine stabilized sialic acid (N2H2S2) in DMSO-AA at 4°C, 23°C, 37°C, and 65°C; (c) Increase of N2H2S from hydrolysis of N2H2S2 in DMSO-AA; (d) No change on N2H2S due to the stabilized N2H2S2 with p-Toluidine; (e) Increase of N2H2 due to hydrolysis of N2H2S and N2H2S2 in DMSO-AA at different temperature; (f) Increase of N2H2 due to hydrolysis of N2H2S and N2H2S2 incubated 0.1% TFA from 2h to 24h (23°C). Triplicate experiments were conducted.