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. Author manuscript; available in PMC: 2017 Sep 5.
Published in final edited form as: Chem Biol. 2012 Apr 20;19(4):449–455. doi: 10.1016/j.chembiol.2012.01.018

Figure 3. ELISA Screening and Characterization.

Figure 3

(A) The initial screen of the supercharged scFV variants for binding. ScFVs were added to the antigen-bound plate at a concentration of 5 μg/ml.

(B) Samples of constructs shown to bind in the initial screen were stored at 70°C for 1 hr, allowed to cool to room temperature, and assayed again to determine which constructs retained activity.

(C) A dilution-series ELISA comparing wild-type and K-pos-1 binding before and after incubation at 70°C for 1 hr.

(D) A dilution-series ELISA comparing the function of wild-type and K-pos-1 in 50% serum. Error bars represent SD calculated from the OD450 values of three identically-prepared wells.

See also Figures S2 and S3.