Table 1.

Screening of Supercharged Single-Chain F_V Variants

Name	Charge	Rosetta Score(Δ Wild-Type)	Mutation Count	Expressiona	Binding	Thermal Resistance
K-neg-4	−19.5	−6.2	24	+ +	No
K-neg-3	−13.5	−9.3	18	+ +	Yes (weak)	No
K-neg-2	−8.5	−8.6	14	+ +	Yes (strong)	No
K-neg-1	−0.5	−5.1	7	+	No
wt	7.5	0	0	+ + +	Yes (strong)	Yes (trace)
K-pos-1	15.5	−4.9	8	+ +	Yes (strong)	Yes (strong)
K-pos-2	18.5	−8.9	11	+	Yes (strong)	Yes (medium)
K-pos-3	22.5	−10.1	14	+	Yes (medium)	Yes (medium)
K-pos-4	29.5	−11	21	+	No
K-pos-5	33.5	−7.9	25	(cytosol)	No

wt, wild-type.

See also Figure S1 and Supplemental File 1.

^aRelative expression levels are indicated by +, ++, and +++ indicating low, medium, and high, respectively.