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PISA of nine human VH/K-His fragments from a transgenic mouse library (12). (A) Analysis with biotinylated P-BSA, followed by HRP-linked streptavidin. (B) The same array after treatment with stripping buffer and re-analysis with HRP-linked anti-human κ chain. Wells 1–5, non-antigen binding VH/K-His fragments from clones of the transgenic mouse library; P5-8, -10, -16, -17, specific anti-progesterone VH/K-His fragments from the same library; Con, TNT mixture control lacking PCR DNA.
