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. 2001 Aug 1;29(15):e74. doi: 10.1093/nar/29.15.e74

Figure 2.

Figure 2

Two-step PCR amplification of mtDNA isolated from six individual muscle fibres. Representative gels of each round of amplification are depicted. (A) An aliquot of 1 µl total DNA template released by lysis of six individual fibres (1–6) was amplified (50 µl PCR reaction) by primer pair SC-D (Table 1). The expected size of this product was 1905 bp. (B) Amplified product (2 µl) from the first round of amplification was used as template in a second round of PCR with primer pair 12F/12R (Table 2). The expected size of this product was 661 bp. Appropriate controls are shown for each round of amplification. +ve, 500 ng ρ+ DNA; –ve, template omitted from PCR reaction; LB, 2 µl aliquot of the single cell lysis buffer used in the DNA extraction; M, DNA size standard with sizes (bp) on the left.