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Quantification of the 7587C mutant allele in single muscle fibres expressing either a COX-positive or COX-negative phenotype. PCR and RFLP were performed as described in Materials and Methods and labelled products were separated through an 8% non-denaturing polyacrylamide gel. A schematic of the HindII digestion pattern expected in both wild-type and mutant mtDNA is shown on the left. Sizes of expected fragments (bp) are shown on the right of the gel.
