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Detection of T7587C mtDNA heteroplasmy by sequencing individual muscle fibres. Products (2 µl) from the first round amplification of single cell DNA isolates by primer pair SC-D (Table 1) were used as template in both the PCR–RFLP analysis of T7587C mutation load and amplification with sequencing primer pair 12F/12R (Table 2). Comparative sequence chromatograms and RFLP gels are shown together. The mutated base is shown (*).
