Table 1. Oligonucleotide primer pairs used for the primary PCR amplification of mtDNA from single cell lysates.
| Forward primer |
Nucleotide positions |
Reverse primer |
Nucleotide positions |
Product size (bp) |
Template for stage 2 PCRa |
| SC-A-for | 503–520 | SC-A-rev | 2484–2463 | 1982 | 1–3 |
| SC-B-for | 2364–2386 | SC-B-rev | 4249–4228 | 1886 | 4–6 |
| SC-C-for | 4155–4175 | SC-C-rev | 6220–6199 | 2066 | 7–9 |
| SC-D-for | 6113–6133 | SC-D-rev | 8017–7996 | 1905 | 10–12 |
| SC-E-for | 7925–7944 | SC-E-rev | 9884–9863 | 1960 | 13–15 |
| SC-F-for | 9767–9784 | SC-F-rev | 11748–11727 | 1982 | 16–18 |
| SC-G-for | 11614–11635 | SC-G-rev | 13638–13617 | 2025 | 19–21 |
| SC-H-for | 13539–13559 | SC-H-rev | 15431–15409 | 1893 | 22–24 |
| SC-I-for | 15331–15350 | SC-I-rev | 836–815 | 2075 | 25, D1–D3 |
All nucleotide positions shown refer to the original Cambridge Reference Sequence report (1).
aSee Table 2 for details of the primers used in the secondary PCR.