Table 2. M13-tagged oligonucleotide primer pairs used for the secondary PCR amplification of mtDNA templates.
Forward primer |
Nucleotide positions |
Reverse primer |
Nucleotide positions |
Product size (bp) |
1F | 516–534 | 1R | 1190–1172 | 675 |
2F | 1138–1156 | 2R | 1801–1782 | 664 |
3F | 1756–1776 | 3R | 2444–2426 | 689 |
4F | 2395–2415 | 4R | 3074–3054 | 680 |
5F | 2995–3013 | 5R | 3645–3627 | 651 |
6F | 3536–3553 | 6R | 4239–4219 | 704 |
7F | 4184–4202 | 7R | 4869–4852 | 686 |
8F | 4832–4849 | 8R | 5570–5551 | 739 |
9F | 5526–5545 | 9R | 6188–6171 | 663 |
10F | 6115–6134 | 10R | 6781–6761 | 667 |
11F | 6730–6750 | 11R | 7398–7379 | 669 |
12F | 7349–7369 | 12R | 8009–7990 | 661 |
13F | 7960–7979 | 13R | 8641–8621 | 682 |
14F | 8563–8581 | 14R | 9231–9212 | 669 |
15F | 9181–9198 | 15R | 9867–9848 | 687 |
16F | 9821–9841 | 16R | 10516–10497 | 696 |
17F | 10394–10414 | 17R | 11032–11013 | 639 |
18F | 10985–11004 | 18R | 11708–11689 | 724 |
19F | 11633–11651 | 19R | 12361–12341 | 729 |
20F | 12284–12302 | 20R | 13005–12987 | 722 |
21F | 12951–12969 | 21R | 13614–13595 | 664 |
22F | 13568–13587 | 22R | 14276–14258 | 709 |
23F | 14227–14246 | 23R | 14928–14911 | 702 |
24F | 14732–14752 | 24R | 15419–15400 | 688 |
25F | 15372–15391 | 25R | 16067–16048 | 696 |
D1F | 15879–15897 | D1R | 16545–16526 | 667 |
D2F | 16495–16514 | D2R | 389–370 | 446 |
D3F | 315–332 | D3R | 803–786 | 489 |