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. 2001 Aug 1;29(15):e74. doi: 10.1093/nar/29.15.e74

Table 2. M13-tagged oligonucleotide primer pairs used for the secondary PCR amplification of mtDNA templates.

Forward primer
Nucleotide positions
Reverse primer
Nucleotide positions
Product size (bp)
1F 516–534 1R 1190–1172 675
2F 1138–1156 2R 1801–1782 664
3F 1756–1776 3R 2444–2426 689
4F 2395–2415 4R 3074–3054 680
5F 2995–3013 5R 3645–3627 651
6F 3536–3553 6R 4239–4219 704
7F 4184–4202 7R 4869–4852 686
8F 4832–4849 8R 5570–5551 739
9F 5526–5545 9R 6188–6171 663
10F 6115–6134 10R 6781–6761 667
11F 6730–6750 11R 7398–7379 669
12F 7349–7369 12R 8009–7990 661
13F 7960–7979 13R 8641–8621 682
14F 8563–8581 14R 9231–9212 669
15F 9181–9198 15R 9867–9848 687
16F 9821–9841 16R 10516–10497 696
17F 10394–10414 17R 11032–11013 639
18F 10985–11004 18R 11708–11689 724
19F 11633–11651 19R 12361–12341 729
20F 12284–12302 20R 13005–12987 722
21F 12951–12969 21R 13614–13595 664
22F 13568–13587 22R 14276–14258 709
23F 14227–14246 23R 14928–14911 702
24F 14732–14752 24R 15419–15400 688
25F 15372–15391 25R 16067–16048 696
D1F 15879–15897 D1R 16545–16526 667
D2F 16495–16514 D2R 389–370 446
D3F 315–332 D3R 803–786 489