. 2017 Jul 1;11(7):DC32–DC36. doi: 10.7860/JCDR/2017/24810.10277

[Table/Fig-1]:

Primers and thermal conditions in genotyping of H. pylori clinical isolates.

Region amplified	Primer sequence (5′ to 3′)	Size of amplicon	PCR cycles
glmM-F	AAGCTTTTAGGGGTGTTAGGGGTTT	294 bp	Initial denaturation at 95 °C for 5 min, followed by 35 cycles of denaturation at 95 °C for 45 sec, annealing at 50 °C for 45 sec and extension at 72 °C for 35 sec. The final extension was at 72 °C for 10 min.
glmM-R	AAGCTTACTTTCTAACACTAACGC	294 bp
oipA-F	GTTTTTGATGCATGGGATTT	401 bp	5 min predenaturation at 95 °C, followed by 35 cycles of 35 sec at 95 °C, 35 sec at 53 °C and 35 sec at 72 °C
oipA-R	GTGCATCTCTTATGGCTTT	401 bp
*sabA-F*	CCGCTAGTGTCCAGGGTAAC	364 bp	35 sec at 95 °C, 35 sec at 50 °C and 35 sec at 72 °C
*sabA-R*	CACCGCGATTTGCGTTGGTA	364 bp	35 sec at 95 °C, 35 sec at 50 °C and 35 sec at 72 °C
babA2-F	AATCCAAAAAGGAGAAAAATATGAAA	832 bp	35 sec at 95 °C, 35 sec at 55 °C and 45 sec at 72 °C. The final extension was at 72 °C for 5 min.
babA2-R	TGTTAGTGATTTCGGTGTAGGACA	832 bp

F: Forward primer, R: Reverse primer