Fig. 2.

The Brh1 genomic region in barley and the syntenic regions in rice, sorghum and brachypodium. a. Brh1 has been mapped between markers CDO545 (green, NCBI accession numbers AA231869.1 and AA231713.1) and BDC129 (yellow), R3139 (blue, AU082160, D25093) and MWG2074 (red, AJ234754.1, AJ234755.1) [21]. MWG2074 was shown to have two loci, the B locus (middle, red) was co-segregating with Brh1. Marker sequences were used to identify matching loci in rice, sorghum and brachypodium (color codes kept). No obvious hits to orthologous sequences could be found for marker MWG2074 in rice and sorghum, or for R3139 in brachypodium. b. Gene content of the Brh1 syntenic region in brachypodium as identified in Fig. 2a. The loci (Bradi1g50590 and Bradi1g50660) matching markers CDO545 and MWG2074 are highlighted green and red, respectively. The genes flanked by loci Bradi1g50590 and Bradi1g50660 are found in the same order in rice and sorghum. The orthologous barley genes were used to screen pooled BAC libraries