Table 3.

Comparison of alpha diversity measures among Meconium samples using the approximative Fisher–Pitman test stratified by individual sample IDs with 9999 permutations

	Observed	Shannon	Simpson	Fisher
Card vs. tube	0.06	1.00	0.27	0.05
Manual vs. EZ1	0.04	0.073	0.23	0.033
RT vs. − 80 °C	0.67	0.59	0.35	0.69
Tube (7 days at − 80 °C)a	0.76	0.22	0.13	0.81
Card (3–5 days)a	0.84	0.53	0.26	0.83
Card (7 days RT)a	0.69	0.25	0.18	0.61
Card (7 days at − 80 °C)a	0.14	0.93	0.44	0.14
Card (3–5 days)b	0.47	0.35	0.90	0.42
Card (7 days RT)b	0.44	0.91	0.89	0.41
Card (7 days at − 80 °C)b	0.13	0.93	0.44	0.14

^aComparison with tube at 0–2 days

^bComparison with tube 7 days at − 80 °C

Values italicized are signficiant with a value of p<0.05