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. 2017 Sep 5;10:409. doi: 10.1186/s13071-017-2353-8

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Co-IP assays further indicate that MNh can bind to TMEM63A and MCh can bind to TMEM147. Lane Input (a, b, c, d): Cell lysates were precipitated with rat anti-TMEM63A-NO IgG, rat anti-MNh IgG, rat anti-TMEM147-O IgG and rat anti-MCh IgG, respectively. Lane IP (a, b, c, d): Cell lysates were precipitated with rat anti-MNh IgG, rat anti-TMEM63A-NO IgG, rat anti-MCh IgG and rat anti-TMEM147-O IgG, respectively. Lane IgG (a, b, c, d): Cell lysates were precipitated with normal rat IgG. IP: immunoprecipitation. Immunoblot analysis using rat anti-MNh IgG and rat anti-MCh IgG demonstrated that rMNh can bind to TMEM63A and rMCh can bind to TMEM147. Lane M: marker