Tab. I.
Primers used in this study for detecting ESBL-encoding genes.
| Targets | Primer Sequences (5'-3') | Annealing Temperature (°C) | References |
|---|---|---|---|
| TEM | ATGAGTATTCAACATTTCCG GACAGTTACCAATGCTTAATCA |
50 | 19 |
| TEM (Sequencing) | TAACCATGAGTGATAACACT
CCGATCGTTGTCAGAAGTAA |
50 | 19 |
| SHV | CTTTACTCGCCTTTATCG TCCCGCAGATAAATCACCA |
50 | 19 |
| SHV (Sequencing) | ACTGCCTTTTTGCGCCAGAT
CAGTTCCGTTTCCCAGCGGT |
56 | 19 |
| CTX-M-1 group | ATGGTTAAAAAATCACTGCGTC
TTGGTGACGATTTTAGCCGC |
55 | 20 |
| CTX-M-2 group | ATGATGACTCAGAGCATTCG
TGGGTTACGATTTTCGCCGC |
55 | 20 |
| CTX-M-8 group | ACTTCAGCCACACGGATTCA
CGAGTACGTCACGACGACTT |
55 | 20 |
| CTX-M-9 group | ATGGTGACAAAGAGAGTGCA
CCCTTCGGCGATGATTCTC |
55 | 20 |
| CTX-M-25 | CACACGAATTGAATGTTCAG
TCACTCCACATGGTGAGT |
50 | 21 |