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. 2017 Sep 4;214(9):2523–2533. doi: 10.1084/jem.20161427

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Figure 1. — STAT3 LOF and STAT1 GOF cells have enhanced pSTAT1 that is partially dependent on impaired induction of SOCS3. (A) pSTAT1 (left) and pSTAT3 (right) in PBMCs after stimulation with IL-6, IL-21, or IL-27 for 15 min. (B) Total STAT1 in PBMCS before stimulation (left) and after cytokine stimulation (right) for 15 min. (C and D) SOCS3 and SOCS1 mRNA (C) and SOCS3 protein (D) levels in naive CD4 T cells after stimulation with IL-6, IL-21, or IL-27 for 1 h. (E and F) Naive (CD45RO⁻) CD4⁺ T cells were transfected with SOCS3–GFP or GFP–control or SOCS3–RNAi or scramble RNAi–control. IL-27 was added 5 h after transfection, and pSTAT1 levels were analyzed 18–24 h later. One-way ANOVA (A–D) and paired and Student’s t tests (E and F) were performed. *, P < 0.05; **, P < 0.01. Data are representative of three independent experiments, unless otherwise indicated on the graph; graphs show means ± SEM. MFI, mean fluorescence intensity.