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. 2017 Sep 4;214(9):2629–2647. doi: 10.1084/jem.20161855

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© 2017 Karmaus et al.

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Figure 7. — mTORC1 regulates one-carbon metabolism to support myelopoiesis. (A) GAM analysis of nodes (n = 3–4 mice per group). (B) GAM analysis of optimally solved edges (n = 3–4 mice per group). (C) Flow cytometry analysis of CD16/32 and CD34 on BM Lin^– LK cells from mice treated with vehicle (PBS) and 5-FU (25 mg/kg). (D) Expression of CD115 on myeloid progenitor cell populations from mice treated with vehicle (PBS) and 5-FU, with mean fluorescence intensity (MFI) plotted within graphs. (E) Expression of CD115 on Lin^– BM cells from mice treated with vehicle (PBS) and 5-FU after liquid culture with M-CSF (10 ng/ml) for 2 d, with MFI plotted above graph. (F) Flow cytometry analysis (left) of F4/80 and CD11b, frequency (middle), and number (right) of CD11b⁺F4/80⁺ in Lin^– BM cells from mice treated with vehicle (PBS) and 5-FU after liquid culture with M-CSF (10 ng/ml) for 2 d. (G) Flow cytometry analysis of CellTrace (left) and CD115 (right) within the gated CD11b⁺F4/80⁺ cells from cultured (M-CSF, 10 ng/ml, 2 d) Lin^– BM cells from mice treated with vehicle (PBS) and 5-FU, with MFI plotted within graphs. (H) Flow cytometry analysis of CD16/32 and CD34 on BM Lin^– LK cells from mice treated with vehicle (DMSO) and MTX (32 mg/kg). (I) Expression of CD115 on myeloid progenitor cell populations from mice treated with vehicle (DMSO) and MTX, with MFI plotted within graphs. (J) Expression of CD115 on Lin^– BM cells from mice treated with vehicle (DMSO) and MTX after culture with M-CSF (10 ng/ml) for 2 d, with MFI plotted within graph. (K) Flow cytometry analysis (left) of F4/80 and CD11b and number (right) of CD11b⁺F4/80⁺ cells in Lin^– BM cells from mice treated with vehicle (DMSO) and MTX after culture with M-CSF (10 ng/ml) for 2 d. Numbers indicate percentage of cells in gates. Data are mean ± SEM. Data are one experiment (A and B) or representative of three experiments (C–K).