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. 2017 Sep 4;214(9):2795–2810. doi: 10.1084/jem.20161955

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Figure 3. — Antigen-specific T cell activation and Tfh cell development require B7 on DCs but not B cells. (A) B7 cKO strains (CD45.2) were adoptively transferred with CFSE-labeled OT-II T cells (CD45.1) and immunized with NP-OVA/Alum 1 d later. Spleens were harvested on day 10, and CFSE dilution was analyzed by flow cytometry. Dashed-line and shaded histograms indicate transferred OT-II T cells in unimmunized and immunized mice, respectively. Data are representative of three independent experiments. (B–E) OT-II cell transfer was done as described in A but without CFSE staining. Recovered total OT-II cell number (B), FACS analysis of Tfh (CXCR5^high PD-1^high) OT-II cells (C), frequency of Tfh cells among OT-II (D), and total OT-II Tfh cell number were analyzed at days 8–10 after immunization (E). Data presented are the combined result of four independent experiments (mean ± SEM). The total numbers of mice in the four combined experiments are B7.1^flox, n = 8; B7 DKO, n = 9; DC-B7 cKO, n = 8; and BC-B7 cKO, n = 11. Statistical significance was determined by one-way ANOVA followed by Dunnett’s multiple comparison.