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. 2017 Sep 4;216(9):2911–2926. doi: 10.1083/jcb.201605119

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© 2017 Capurro et al.

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Figure 9. — GPC6 stimulates the migration of Smo to the cilia after Shh treatment. (A) NIH 3T3 cells were transfected with GPC6, GPC6ΔGAG, or vector control. Transfected cells were treated with Shh for the indicated time periods, and cells were stained with antibodies against Smo and acetylated tubulin. The fluorescence intensities shown represent the mean of measurements performed in the following number of cells transfected with vector/GPC6/GPC6ΔGAG: 23/29/23 (0 h), 27/26/19 (1 h), 26/26/28 (2 h), and 26/37/22 (4 h) ± SD. *, P < 0.01 (significantly different). (B) NIH 3T3 cells were transfected with GPC6 or GPC6ΔGAG. Transfected cells were treated with SAG for the indicated time periods, and cells were stained with antibodies against GPC6 and acetylated tubulin. The fluorescence intensities shown represent the mean of measurements performed in the following number of cells transfected with GPC6/GPC6ΔGAG: 9/7 (0 h), 15/15 (0.5 h), 11/11 (2 h), and 10/10 (4 h) ± SD. Two-tailed Student’s t tests were applied for statistical analysis.