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. 2017 Sep 4;216(9):2979–2989. doi: 10.1083/jcb.201607048

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© 2017 Cantaut-Belarif et al.

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Figure 3. — Short-term stimulation of microglia decreases the proportion of synaptic swapping trajectories. (A and B) SC of swapping GlyR α1 (A) and GABA_AR γ2 (B) in control (black) and after LPS application (gray). (C and D). Transition frequency of GlyR α1 QDs (C) and GABA_AR γ2 QDs (D) in and out of the synapses. Mean ± SEM; ns, P > 0.05; **, P < 0.01; t test. (E) Representative Western blots of GlyR α1 and GABA_AR γ2 in the total amount of protein (input) or in the biotinylated fraction at the cell surface (biotin) in control of LPS-treated cells. (F) Mean ratio (±SEM) of surface-to-total GABA_AR γ2 and GlyR α1 levels in control condition (white) and after LPS application (gray). Data are expressed as a percentage of control (n = 5 and 4 cultures, respectively; ns, P > 0.05; t test).