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. 2017 Jan 10;8(31):50447–50459. doi: 10.18632/oncotarget.14579

Figure 2. Immunoproteasome subunit LMP7 is essential for development of colitis-associated cancer (CAC).

Figure 2

A. CAC was induced in WT and lmp7−/− mice and the change in weight was monitored in naïve and AOM/DSS-treated mice over a period of 75 days. Data represent mean ± SEM (n=10-12 mice per group). ***P<0.001. B. Representative images of H&E- and PAS-stained colon sections of control or AOM/DSS-treated mice (day 80). Scale bars: 100µm for H&E and 200µm for PAS staining. C. Colonic tumor incidence in naïve and AOM/DSS treated mice (day 80). Data represent mean ± SEM (n=10-12 mice per group). ***P<0.001. D and E. Reduced NF-κB levels in LMP7 deficient mice after induction of CAC. Immunoblot analysis for p105/p50 (D), p65 and c-Rel (E) was performed for the whole colonic tissue of AOM/DSS-treated mice (day 80). α-Tubulin or β-Actin served as a loading control. A representative of two experiments is shown. F. Densitometric analysis for colonic p65 expression on day 80 after induction of CAC in WT and lmp7−/− mice. Data represent mean ± SEM of two independent experiments and are normalized to p65 expression in the colon of naïve WT mice. **P<0.01.