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. 2017 Sep 4;216(9):2813–2826. doi: 10.1083/jcb.201610019

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© 2017 Suresh et al.

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Figure 2. — Nup2 mediates accurate mitotic segregation of NPC proteins to daughter nuclei. (A) Live-cell imaging of the INM protein CR-MtgA and the transmembrane NPC protein Ndc1-GFP in WT (SGS317) and (B) Δnup2 cells (SGS320-H) during mitosis, mitotic exit, and G1. Arrows indicate the predicted location of the nucleolus. The images have been overexposed to show the signal around the nucleolus. (C) Quantitation of the mean intensity of Ndc1-GFP at the NE defined by the INM protein CR-MtgA at G2 and then G1 from time-lapse imaging (SGS317 and SGS320-H). ****, P < 0.0001. Error bars represent ± SD. (D) Live-cell imaging of the peripheral NPC protein Nup49-GFP in relation to NLS-dsRed in WT cells (SM112) and Δnup2 cells (SM118-H; E) during G2-M-G1 transitions. Arrows indicate the predicted location of the nucleolus. Bars, ∼5 µm.