Figure 9.

TRIM37 or PEX5 depletion induces apoptosis and results in increased sensitivity to oxidative stress. (A and C) Lysates from HepG2 control versus PEX5 KD cells and control versus TRIM37 KD cells were immunoblotted for the indicated proteins. (B and D) HepG2 control versus PEX5 KD cells or control versus TRIM37 KD cells were either left untreated or were treated with 0.25 mM H₂O₂ (B) or the indicated H₂O₂ concentrations (C) for 3 h before propidium iodide staining for flow cytometry and cell death analysis. Data from triplicate samples in each group are presented as mean ± SEM and are representative of three independent experiments; *, P < 0.05; **, P < 0.01 (Student’s t test). (E and F) HepG2 control versus TRIM37 KD cells and normal versus patient fibroblast cells were immunostained with antibody that detects cleaved Caspase 3. (G) Cleaved Caspase 3 and TRIM37 levels in lysates from normal and patient cells (AG02122). Bars, 10 µm.