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. 2017 Sep 4;216(9):2715–2729. doi: 10.1083/jcb.201701154

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© 2017 Hau et al.

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Figure 4. — MEIS recruits PARP1 to Dcx(−2.7). (A and B) ChIP-qPCR for the proteins indicated at 5 h of differentiation in cells transfected with Meis1/2-specific siRNAs (gray bars) or nontargeting control siRNAs (black bars): MEIS, PARP1, and PBX1 at Dcx(−2.7) (A), and H1 at Dcx(−2.7) (B). (C) ChIP-qPCR for H1 at Dcx(−2.7) in undifferentiated cell cultures. H1 is not released from the Dcx promoter/enhancer when cells are subjected to the cellular differentiation protocol under Meis-knockdown conditions. The asterisks indicate statistically significant enrichment of ChIP with the antibodies indicated relative to ChIP with the IgG control antibodies for the same conditions, with *, P < 0.05; **, P < 0.01; ***, P < 0.001. Statistical significance of ChIP results between experimental groups is given as p = numerical value. ChIP data are represented as means ± SEM. Samples sizes and the number of biological replicates are listed in Table S4.