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. 2017 Apr 6;8(31):50655–50664. doi: 10.18632/oncotarget.16901

Figure 2. Usp5 knockdown suppressed cell growth and induced apoptosis in HepG2 cells.

Figure 2

(A) Usp5 expression was suppressed by siUsp5-1 and siUsp5-2 in HepG2 cells. (B and C) siUsp5 suppressed the cell viability at day 3 (B) and induced the G1-phase arrest in HepG2 cells (C). (D) the representative images and statistical analyses of colony formation assays in siUsp5 transfected HCC cells. (E) siUsp5 induced more apoptotic cells of HepG2 cells by flow cytometry analyses. Top, results of one such assay; and bottom, mean ± SD of three independent experiments. *P < 0.05; **P < 0.01.