Figure 4. Usp5 overexpression promoted tumorigenicity in vivo.

HepG2 cells transfected with pUsp5 or pCDNA were injected s.c. into nude mice. (A) the growth curves of tumor volumes were measured. Each data point represented the mean ± SD of five mice. (B) pUsp5-transfected cells generated bigger tumors than their control cells. (C) the immunofluorescence of Ki-67 and Usp5 stained sections followed by counterstaining with DAPI. **P < 0.01.