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. 2017 Sep 4;216(9):2877–2889. doi: 10.1083/jcb.201705167

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© 2017 Shutova et al.

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Figure 2. — Organization of stress fibers and distribution of NMIIA and NMIIB in COS-7 cells after ectopic expression of NMIIB or NMIIA. (A–C) Wild-type cells (A) or cells expressing GFP-NMIIB (B) or GFP-NMIIA (C) fixed and stained with phalloidin (magenta) and NMIIA (cyan) or NMIIB (yellow) antibody. Left panels show xy maximum projections, whereas right panels show z slices along yellow lines. Bars: (xy panels) 20 µm; (z panels) 5 µm. (D) Percentage of cells containing indicated types of stress fibers in groups of cells expressing low, medium, and high levels of GFP-NMIIA (n = 119 cells) or GFP-NMIIB (n = 93 cells). (E) Wild-type cells (left) or cells expressing GFP-NMIIB (middle) or GFP-NMIIA (right) fixed and stained with phalloidin (magenta) and vinculin (green). Bar, 20 µm.