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. 2001 Aug 15;29(16):3448–3457. doi: 10.1093/nar/29.16.3448

Figure 2.

Figure 2

Effects of configuration around the HS2 region on the enhancer activity in transient expression assay. (A) Effects of replacement of curved DNA with non-curved DNA on the enhancer activity. Either or both the nucleosome regions of DNA bend sites, ɛB-16 and ɛB-17, or HS2 site were replaced with fragments of identical length derived from pBR322 (see Fig. 1 legend). (B) Effects of distance between ɛB-16 and NF-E2 sites on the enhancer activity. The distance between ɛB-16 and NF-E2 sites was changed by deleting or inserting fragments of various lengths into the nucleosome linker region next to ɛB-16. (C) Effects of the orientation of the original and modified HS2 regions on the enhancer activity. The constructs HS2R, +80R, +100R, +170R and Δb-16R contained the same fragments as in HS2, +80, +100, +170 and Δb-16 in the reverse orientation, respectively. Solid Tc boxes and hatched boxes indicate the non-curved DNA fragment and Aγ-globin promoter, respectively. The luciferase activities shown are the mean values of triplicate assays, and the horizontal bars represent standard deviations.