Skip to main content
. 2001 Aug 15;29(16):3448–3457. doi: 10.1093/nar/29.16.3448

Figure 6.

Figure 6

Effect of NF-E2 on nucleosome phases at the HS2 region. The plasmid ±0 construct was introduced into HeLa cells with or without the plasmids (exNF-E2) expressing NF-E2 subunits, p18 and p45. HeLa cells were harvested 24 h after transfection and subjected to RT–PCR (A) and in vivo DNase I footprinting assay (B). G3PDH expression was examined as a control in RT–PCR. Horizontal arrows and a square bracket indicate the periodic 10-bp cleavage sites of the nucleosome indicating rotational phases and a region with higher sensitivity found in the cells with exNF-E2, respectively. The DNA fragments showing increased sensitivities were marked by a closed triangle (at the NF-E2 binding site) or dots (at the other sites)..