Figure 2. Annexin-V/PI staining showed that CUR treatment induced apparent apoptosis in HEp-2, SCC-15, FaDu cells.
(A–C) Annexin-V/PI staining showed that 10 μM CUR treatment for 36 h induced apparent apoptosis in cell lines. (D–F) After treatment with CUR, the cells lines showed apoptosis at rates of 17.20%, 19.54% and 21.60%, respectively. Cells lines were treated with CUR in the presence or absence of the ATM inhibitor KU55933 for 24 h, followed by assessment for cell viability using MTT assays. After treatment with KU55933, the apoptosis rate was significantly reduced, not only in HEp-2 cells group, but also in SCC-15 and FaDu cells group.