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. 2017 Apr 13;8(31):50747–50760. doi: 10.18632/oncotarget.17096

Figure 4. CUR-induced apoptosis is associated with activation of ATM/Chk2/p53 signal pathway.

Figure 4

(A–B) HEp-2 cells were treated with CUR (10 μM) for the indicated times and harvested. CUR triggered a significant increase in pATM levels in HEp-2 cells. (C–D) Examination of Chk2 showed a marked increase in phosphorylation in response to CUR. (E) The mRNA expression of ATM and Chk2 was borderline decreased by CUR pretreatment of HEp-2 cells (p > 0.05). (F–G) Western blot analysis using antibodies specific against p53 and p21 showed a marked increase in protein state of p53 and p21 in CUR treated HEp-2 cells. And CUR pretreatment of HEp-2 cells decreased Cdk1 expression level at 12 h, with increasing effect at 24 h. Results are mean ± SD of three independent experiments (n = 3; P < 0.005).