Figure 1. Ascites promoted ovarian cancer cell migration in a TGF-β-dependent manner.

(A) Cells were seeded on transwell and migrated cells were stained and quantified after treatment with the ascites or inactivated ascites (heated at 100°C for 10 min) for 10 hours. (B) The level of activated TGF-β in seven different ascites specimens (#1-#7) was measured with ELISA kit. Neg, negative control. (C) Same as (A), after 10-hour treatment with TGF-β, ascites or ascites conbined with anti-TGF-β antibody, migrated cells were quantified. The data showed the means ± SD from three independent experiments. *p < 0.05 vs corresponding control.