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. 2017 May 10;8(31):51177–51189. doi: 10.18632/oncotarget.17750

Figure 4. GC cells proliferation and invasion abilities were mediated by neogenin.

Figure 4

(A) The expression levels of netrin-1 receptors, including UNC5A-D, neogenin, DCC and DSCAM were detected by qRT-PCR. N.D., not detected. (B) UNC5B and neogenin protein expression levels were analyzed in GC cell lines by western blotting. (C) HGC27 cells were transfected with control, UNC5B, or neogenin siRNA. Protein expression levels were measured by western blotting analysis. (D) CCK-8 assay showed that neogenin silencing suppressed cells proliferation in HGC27 cells. (E, F) Neogenin knockdown restrained cells invasion in Matrigel-coated Transwell. The number of invasive cells were quantified. Original magnification, ×100; Scale bar = 100 μm. (G) HGC27 cells were transfected with control, netrin-1, or neogenin siRNA. Protein expression levels were measured by western blotting analysis. (H–J) HGC27 cells proliferation and invasion abilities were assessed by using CCK-8 and Matrigel-coated Transwell assays. The combination of netrin-1 and neogenin siRNA significantly suppressed cells proliferation and invasion. The number of invasive cells were quantified. Original magnification, ×100; Scale bar = 100 μm. *p < 0.05, **p < 0.01, ***p < 0.001.