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. 2017 May 10;8(31):51177–51189. doi: 10.18632/oncotarget.17750

Figure 5. In vivo analysis netrin-1 in regulation of GC cells growth and metastasis.

Figure 5

(A) After being transfected with lentivirus, HGC27 Scr and shNTN1-1 cells were injected into flanks of nude mice, and the mice were sacrificed after 4 weeks. (B, C) Tumor volumes and tumor weights were examined in HGC27 groups at 5 different time points. (D, E) Xenograft tumors were obtained as in (A), and then the samples were fixed and embedded in paraffin for immunohistochemical staining targeting Ki-67. Ki-67 positive nuclei rates for HGC27 were calculate by Image pro plus. Original magnification, 200×; Scale bar = 100 μm. (F) Photographs of tumors were taken by the IVIS Imaging System (Caliper Life Science, Hopkinton, MA). Representative luciferase signal was captured in each group six weeks after injected into HGC27 cells with netrin-1 knockdown and negative control cells. (G, H) Silencing netrin-1 suppressed gastric cancer metastasis. (G), representative micrographs of liver tissues with metastatic nodules(black arrowhead) were shown by HE staining. Original magnification, 200×; Scale bar = 100 μm.*p < 0.05, **p < 0.01, ***p < 0.001.