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. 2017 May 10;8(31):51177–51189. doi: 10.18632/oncotarget.17750

Figure 6. Effects of netrin-1 and neogenin on PI3K/AKT signaling pathways.

Figure 6

(A) Effect of netrin-1 inhibition and overexpression regulated the expression level of p-FAK/FAK, p-AKT/AKT and MMP-9 in HGC27, AGS, BGC823 and MKN45 cells, respectively. (B) After being transfected with neogenin siRNA, the expression of p-FAK/FAK, p-AKT/AKT and MMP-9 were analyzed by western blotting in BGC823 cells. (C) CCK-8 assay showed that neogenin silencing suppressed cells proliferation in BGC823 cells with netrin-1 overexpression. (D) The proliferation ability of GC cells were suppressed in BGC823 cells with LY294002-treated by CCK-8 assay. (E, F) Neogenin knockdown restrained BGC823 cells invasion in Matrigel-coated Transwell. The number of invasive cells were quantified. Original magnification, ×100; Scale bar = 100 μm. (G, H) LY294002 inhibited BGC823 cells invasion in Matrigel-coated Transwell. The number of invasive cells were quantified. Original magnification, ×100; Scale bar =100 μm. *p < 0.05, **p < 0.01, ***p < 0.001.