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. 2017 Apr 20;8(31):51296–51316. doi: 10.18632/oncotarget.17261

Figure 5. PEITC exerts a synergistic effect on the cisplatin-induced reduction of MCF-7 cell viability.

Figure 5

(A) HEK293T cells were incubated with PEITC (15 μM), ML323 (30 μM) or with DMSO (vehicle only) in the presence and absence of hydroxyurea (2.5 mM) for 3 h. Whole cell lysates were analyzed by SDS-PAGE and western blot. (B) MCF-7 cells were incubated with PEITC, cisplatin or with a 1:1 combination of PEITC and cisplatin for 48 h. Cell viability was measured using the CCK-8 assay. Data represent quadruplicate samples of at least 3 independent experiments (n ≥ 12). (C-D) MCF-7 cells were treated with 12 or 25 μM PEITC in combination with 7.5 μg/mL cisplatin for 48 h. Inhibition of cell growth/viability was measured using the CCK-8 assay and CI values were determined using the Bliss independence model as described in Materials and Methods. Data represent quadruplicate samples from 3 independent experiments (n = 12). (E). As in (C), drug interaction was analyzed using Combenefit. Data represent quadruplicate samples from 3 independent experiments (n = 12). ‘ns’ denotes not significant. * p < 0.05; ** p < 0.01; *** p < 0.001.