Figure 8. Decitabine/Vorinostat/BGB324 triple combination treatment prolonged the survival and decreased the leukemic burden in mice.
OCI-AML3 luciferase expressing cells were treated with 0.1 μM DAC, 0.75 μM VOR, 0.5 μM BGB324 and the double DV and triple DVB combination in a sequential manner. (A) The viability of OCI-AML3 luciferase cells was assessed using the CellTitre-Glo® assay and represented as percentage of control. Data represent mean ± SEM; n = 3 (***= p < 0.001; **= p < 0.01; *= p < 0.05). (B) 1.5 × 106 OCI-AML3 luciferase cells (treated in vitro with vehicle control, double DV and triple DVB combinations in a sequential manner for 72 hours) were transplanted via tail vein injection into NSG mice (n = 5 per treatment group). Bioluminescence imaging commenced 15 days post transplantation. Representative images taken at day 29 post transplantation of surviving mice show the disease burden in mice that received control (top), double DV (middle) and triple DVB (bottom) treated cells. Average luminescence intensity for each cohort is shown as Photons/sec/mm/sq (C) Mice were sacrificed at the first sign of illness and Kaplan Meier curves were generated comparing the survival of mice that received control treated OCI-AML3 luciferase cells (black line) those that received cells treated with the double DV (blue dashed line) and triple DVB (red dashed line) combination (**= p < 0.01; *= p < 0.05).