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. 2017 May 8;8(31):51447–51461. doi: 10.18632/oncotarget.17691

Figure 5. Inhibition of SIRT1 using EX-527 or SIRT1 siRNA transfection enhanced MCP-1 expression and foam cell formation.

Figure 5

Human THP-1 macrophages were pretreated with EX-527 (2 μM, for 2 hrs) or SIRT1 siRNA (20 μM, for 24 hrs), and then exposed to 80 μg/mL of ox-LDL for an additional 24 hrs. Western blot for SIRT1 and MCP-1 proteins were analyzed from the ox-LDL-stimulated THP-1 cells. β-actin was used as loading control (A-C) and (F-H). THP-1 macrophage-derived foam cell formation was determined using ORO staining method (D-E) and (I-J). Scale bar: 40 μm. Bar graph indicates the mean ± SD (n = 3). *P < 0.05 and **P < 0.01 vs. Cont group (NC siRNA group); #P < 0.05 and ##P < 0.01 vs. EX-527 group (SIRT1 siRNA group); &P < 0.05 and &&P < 0.01 represent significant differences between ox-LDL group (NC siRNA+ox-LDL group) and EX-527+ox-LDL group (SIRT1 siRNA+ox-LDL group).