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. 2017 May 11;8(31):51478–51491. doi: 10.18632/oncotarget.17807

Figure 6. Characteristics of Mcl-1 expression and ployubiquitin following different treatments of 5-FU, DTN and DHA.

Figure 6

(A) HCT116 cells were treated with single or combination treatment of 5-FU, DTN and DHA for 48h. The protein expression of Mcl-1 was detected using western blotting. (B) HCT116 cells were treated with triple combination treatment of 5-FU, DTN and DHA, following the interference of cells by NAC, Spermidine or MG132, ubiquitination of Mcl-1 protein were immunoprecipitated and detected using Western Blot. β-actin was used for loading control. *P <0.05 showed a significant difference compared to control.