Table 3. Characteristic of ROS levels and apoptosis- or autophagy- related proteins.
| Group | Vehicle control | 5-FU+DTN+ DHA | 5-FU+DTN+ DHA +NAC | 5-FU+DTN+ DHA+SP | 5-FU+DTN+ DHA+MG |
|---|---|---|---|---|---|
| ROS levels | 100.81± 4.43 | 178.3±24.19* | 112.3±19.49▲ | 162.85±18.20* | 198.15±24.45* |
| Atg-5 | 12.81 ± 2.11 | 26.31± 2.81* | 48.07±3.79*▲ | 66.09±4.51*▲ | 41.75±5.43*▲ |
| p62/SQSTM1 | 8.26 ± 1.24 | 22.30± 3.44* | 36.23±3.79*▲ | 55.28±5.98*▲ | 33.44±4.93*▲ |
| Caspase-3 | 7.04 ± 1.09 | 33.69± 6.65* | 16.41±2.57*▲ | 16.61±1.98*▲ | 17.08±3.44*▲ |
| Caspase-9 | 6.15± 1.21 | 29.18± 9.12* | 15.08±2.43*▲ | 14.52±2.04*▲ | 14.31±1.33*▲ |
| Ub-Mcl-1 | 6.23± 1.12 | 28.17± 5.08* | 15.51± 3.17*▲ | 16.27± 3.31*▲ | 40.60± 6.49*▲ |
Notes: Caspase-3 indicates the cleaved 17 kD fragments of Caspase-3; Caspase-9 indicates the cleaved 35 kD fragments of Caspase-9, Ub-Mcl-1 indicates the level of Mcl-1 ubiquitination; Data was showed as X¯± Sd, n = 3,. *P <0.05 showed a significant difference compared to control, ▲P <0.05 showed a significant difference compared to treatment of 5-FU+DTN+DHA group.