Figure 2. Normal GT198 expression in mouse tissues.
(A) Whole mount in situ hybridization showing GT198 mRNA stained in blue at mouse embryonic stages of E8.5, E9.5 and E10.5. (B) Immunohistochemical staining of GT198 protein in mouse embryo at stage E12.5. A negative control absence of primary antibody is at the left. (C) GT198 protein expression in developing and adult mouse testes showing nuclear staining in germ cells. (D) Decreased GT198 expression in multiple tissues as indicated in mouse developmental stages E13.5, E15.5, and E18.5. (E) Positive expression in adult mouse brain, thymus, ovary, bone marrow but not in heart. Immunohistochemical staining sections (B-E) are counter-stained with hematoxylin. Scale bars = 1 mm (A-B), 50 μm (C-E).