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. 2017 May 25;8(31):51591–51607. doi: 10.18632/oncotarget.18196

Figure 8. GT198 activation stimulates VEGF and increases U-251 cell survival.

Figure 8

(A) U-251 cells were transfected with GFP-GT198 (1-217), GFP-mutant GT198 (126-217), or together with GT198 siRNA (100 nM) as indicated. (B) GT198 siRNA switched the balance of expression between the wild type (long arrow) and GT198 splice variants (short arrows) in RT-PCR analysis. (C) GT198 siRNA induced cytosolic VEGF isoforms and vWF expression (arrows) in Western blot analysis. Reduced wild type GT198, unchanged nuclear VEGF, and β-actin served as controls. (D) U-251 cells were cotransfected with VEGF promoter-luciferase (100 ng) together with GT198 siRNA. Relative luciferase light units were shown as means of triplicate transfections ± S.E. (n=3). P values shown were calculated by linear progression. (E) U-251 cells were transfected in duplicate with 0 nM (black) or 100 nM GT198 siRNA (red), and treated at indicated concentrations of cytotoxic drug paclitaxel for 72 hours before analysis using MTT cell viability assay. IC50 values are calculated by nonlinear regression sigmoidal curve fit.