Skip to main content
. 2017 Aug 14;114(35):E7291–E7300. doi: 10.1073/pnas.1701791114

Fig. 3.

Fig. 3.

JNK activation and tumor-like growth in the absence of hemocytes. (A) Larval wing primordia of the indicated genotypes, raised at 18 °C to turn off transgene expression in a GAL80-dependent manner, and stained for DAPI (gray), MyrT (red), and dMMP1 (green) or NimC1 (green, Right). (Scale bars, 50 µm.) MyrT is not expressed and dMMP1 is not induced under these circumstances. Arrow points to the endogenous expression of dMMP1 in the trachea and arrowheads point to a few hemocytes recruited to control wing primordia. (B) Cartoon showing the allograft-transplantation protocol. Wing primordia carrying CIN or scrib-i; Ras-V12 transgenes under control of the ap-GAL4 driver, and silenced with the GAL80ts factor, were dissected from L3 larvae raised at 18 °C, cut into small pieces, and injected into the abdomen of young adult females. Fly hosts were then transferred to a temperature of 29 °C to induce transgene expression. (C, E, G, and J) Micrographs of adult flies carrying MyrT-labeled (red) implants of the indicated genotypes and expressing GFP (green) under the he-GAL4 driver. Host flies were maintained at 29 °C to induce transgene expression; images were taken 5 days (G and I) or 12 days (C and E) after implantation; ratios indicating the reproducibility of the phenotype are shown. In E and J, host flies expressed the proapoptotic gene reaper under the control of the he-GAL4 driver. (D, D′, F, F′, and H–L) Allograft transplants of the indicated genotypes stained to visualize GFP (green, D, F, H, I, and K) or NimC1 (red, I, I′, and L), dMMP1 (cyan, D′, F′, H′, K′), and DAPI (green, D, F, H, I, I′, K, and L). Transplants were extracted 5 days (H–L) or 12 days (D–F′) after implantation. (Scale bars, 100 µm.) (M) Histogram plotting the size [in arbitrary units (a.u.)] of preimplanted wing disc pieces (Left) or allograft transplants expressing scrib-i; Ras-V12 and implanted in adult flies expressing GFP (Middle) or GFP and reaper (Right) transgenes. Error bars indicate SD. No statistical difference (ns) in allograft size was observed upon induction of reaper expression in the adult hemocytes [P(0.05) = 0.6007]. Volume (preimplanted wing disc) = 6.17.104 ± 1.3.104 (n = 6). Volume (allograft in he>GFP hosts) = 4.18.107 ± 1.13.107 (n = 14). Volume (allograft in he>rpr hosts) = 4.35.107 ± 1.5.107 (n = 11).